Chromosomes structure and Genome Organization. Structure of Prokaryotic & Eukaryotic Genes, Transposable Genetics Elements, Repetitive elements, LINE and SINES, VNTRs, Central Dogma of Molecular Biology
Cloning strategy, Genetic and physical mapping, molecular markers, PCR Primer & Oligos Designiing,
Human Genome Project, Current techniques: Sanger’s method & Maxam- Gilbert method. Next Generation Sequencing Techniques, Sequence Assembly and Annotation, ORFs, Gene Identification and Prediction Methods, Genome Alignment and Comparative Genomics. Pyrosequencing & its modifications: Solexa, 454 sequencing.
Microarray Basics, DNA Hybridization, DNA & Protein chips. Experimental Design: Features, target Preparation, Labeling & Sensitivity, Spotting media. Microarray Data analysis: image and raw data, Preprocessing & Normalization methods, Pattern Finding & Clustering. Statistical Tests and differential gene expression analysis, Applications of Microarray Technology.
GEO, YMD, SMD, ArrayExpress etc. SNOMAD, magic tool(open software). Bioconductor, Commercial & Open Source tools for microarray.
1) Genes VII, Latest Edition. 2) TA Brown. “Genomes”. 2nd Edition, Bios Scientific Publishers Ltd., 2002. 3) “Microarray Gene Expression Data Analysis: A Beginner's Guide” (ISBN: 978-1-4051-0682-5). 4). Dov Stekel. “Microarray Bioinformatics.” (ISBN:9780521670500).
Links:
[1] https://www.biotech.iisuniv.ac.in/courses/PG-Courses/Bioinformatics/Molecular-Genetics-and-Genomics
[2] https://www.biotech.iisuniv.ac.in/academic-year/2018-2019