Published on Biotechnology (https://www.biotech.iisuniv.ac.in)

Home > Tissue Culture

Tissue Culture [1]

Paper Code: 
AMB 402
Credits: 
03
Contact Hours: 
45
Objective: 

 

This paper will enable students to-
  • learn the fundamental of Plant Tissue Culture
  • get acquainted with various techniques in Plant Tissue Culture
  • analyze the genetic manipulations in Plant Tissue Culture
  • study the industrial application of Plant Tissue Culture
  • understand the basics of Animal Cell Sciences
  • learn the basic requirements for Animal Cell Culture
12.00
Unit I: 
Introduction to Plant Tissue Culture

 

History Laboratory Requirements and General Techniques: Introduction, Techniques and Requirements.Tissue Culture Media: Introduction, medium constituents, media Selection, Media preparation.Cell Culture: Introduction, isolation of single cells, suspension cultures, Culture of single cells, plant cell reactors, application of cell culture
Cellular Totipotency: Cytodifferentiation, Organogenesis Differentiation. Application of cellular totipotency.
8.00
Unit II: 
Embryogenesis

 

Somatic Embryogenesis: Introduction, Induction, development and maturation of Somatic Embryos Large Scale production of Somatic Embryos. Somatic Embryo versus Zygotic Embryos. Synthetic seeds. Applications of Somatic Embryogenesis
In – vitro Pollination and fertilization: Introduction, terminology, In vitro pollination, 
In vitro fertilization, applications.
10.00
Unit III: 
Genetic Engineering in Plants

 

Genetic transformation of plants: Introduction, methods of Gene transfer, selection and identification of transformed cells, Applications of genetic transformation.
Germplasm Storage: Cryopreservation, Introduction, Long – term storage, Short term or medium – term storage. 
Production of Secondary Metabolites: Strategies to optimize product yield, commercial aspects.
7.00
Unit IV: 
Introduction and Growth Requirements for Animal Cell Culture

General concepts of animal cell culture, Serum free media and role of serum in serum media, Growth Kinetics

8.00
Unit V: 
Culture of Animal Cells

 

Primary and established cell line cultures, Concept of differentiation and dedifferentiation
Transfection methods,  Scaling up of animal cells. 
ESSENTIAL READINGS: 

 

1. Advanced Methods in Plant Biotechnology, David R Murray, Panima Publishing Corporation. 1996
2. Plant, Genes and Crop Biotechnology, 2nd Edition, Marten J Chrispeels & David E Sadava, Jones and Barlett Publishers. 1999.
3. Biotechnology in Crop Improvement, H S Chawla, International Book Distributing Company, 1998. 
4. Practical Application of Plant Molecular Biology, R J Henry, Chapman and Hall,1997. 
5. Laboratory Manual for Plant Biotechnology, H S Chawla Oxford & IBH publishing Co. Pvt Ltd. 2003
6. Plant Biotechnology – The Genetic Manipulation of Plants, Adrian Slater, Nigel Scott & Mark Fowler, Oxford University Press. 2003
7. Plant Tissue Culture – Techniques & Experiments, 2nd edition,  Roberta, H Smith, Elsevier, 2000
8. Culture of Animal Cells, 4th  edition,  R  Ian Freshney, Wiley-Liss, 2000
9. Animal Cell Culture-Practical Approach, 3rd edition,  John R.W. Masters, Oxford, 2000
REFERENCES: 

 

1. Molecular Plant Biology- Volume 1 & 2. Philip M Gilmartin and Chris Bowler:  Oxford University Press. 2005
2. Plant Tissue Culture, S S Bhojwani and M.K Razdan,  Elsevier, 2004
3. Plant Biotechnology, J Hammond, P Mc Garvey & V Yusibov, Springer (India) Pvt Ltd. 2000
4. Plant Cell and Tissue Culture for the Production of Food ingredients, J Fu, G Singh, and W R Curtis (Eds), Kluwer Academic/Plenum Press, 1999. 
5. Biotechnology – Secondary Metabolites. K G Ramawat, J M Merllon, Oxford & IBH publishing house Co. Pvt. Ltd. 2000
6. Plant Molecular Biology – A Practical Approach, C H Shaw, IRL Press Ltd. 1988.
7. Basic Cell Culture by J M Davis, Oxford , 2000
Academic Year: 
2018-2019 [2]

Follow Biotechnology on:

IIS(deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158

Source URL: https://www.biotech.iisuniv.ac.in/courses/UG-Courses/B.Sc%28Elective%29/APPLIED-MICROBIOLOGY/TISSUE%20CULTURE

Links:
[1] https://www.biotech.iisuniv.ac.in/courses/UG-Courses/B.Sc%28Elective%29/APPLIED-MICROBIOLOGY/TISSUE%20CULTURE
[2] https://www.biotech.iisuniv.ac.in/academic-year/2018-2019