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PLANT TISSUE CULTURE [1]

Paper Code: 
BTE 501
Credits: 
3
Contact Hours: 
45
Objective: 

Upon completion of the course learners will be able to:

CO 63: Learn the fundamental of Plant Tissue Culture

CO 64: Get acquainted with various techniques in Plant Tissue Culture

CO  65: Study more techniques in Plant Tissue Culture

CO  66:  Study applied aspects in Plant Tissue Culture

CO 67: Learn genetic manipulation techniques in plant tissue culture & their application.

8.00
Unit I: 
Fundamentals
  • Brief history of Plant Tissue Culture (Contribution of Indian Scientist), Introduction to in vitro Methods. Terms and definition.
  • Plant Tissue Culture media.
  • Concept of totipotency
  • Callus Culture
  • Embryo culture, embryo rescue after hybridization and its applications.
  •  Introduction to the processes of embryogenesis and organogenesis and their practical applications.

 

 

10.00
Unit II: 
Various techniques- I
  • Micropropagation
  • Axillary bud, shoot- tip and meristem culture
  • anther culture: Haploids and their application
  • Somaclonal variations and applications.
  • In vitro pollination and fertilization.
  • Endosperm culture and production of triploids.
  • Single cell suspension cultures.

 

 

7.00
Unit III: 
Various techniques- II
  • Introduction to protoplast isolation: Principles and applications.
  • Testing of viability of isolated protoplasts
  • Various steps in the regeneration of protoplasts
  • Various methods for fusing protoplasts: Chemical and Electrical
  • Somatic hybridization

 

 

10.00
Unit IV: 
Applied aspects
  • Use of markers for selection of hybrid cells.
  • Practical applications of somatic hybridization (Hybrids vs. Cybrids)
  • Use of plant cell and tissue culture for genetic manipulation of plants.
  • Marker assisted plant breeding.

 

 

Unit V: 
Genetic manipulation in plants
  • Methods of gene transfer: Direct andAgarobacterium mediated.
  • Introduction of  A. tumefaciens and A. rhizogenes (Monocots vs. Dicots).
  • Formation of hairy root culture. 
  • Practical application of genetic transformation .

 

 

ESSENTIAL READINGS: 
  • Plant Tissue Culture. M. K. Razdan 3rd Edition. OXFORD & IBH PUBLISHING 2019
  • Introduction to Plant Tissue Culture.  H. S Chawla. 3rd Edition. OXFORD & IBH PUBLISHING. 2020
  • Plant Biotechnology – The genetic manipulation of plants. Adrian Slater, Nigel Scott & Mark Fowler:  Oxford University Press. 2003

 

 

REFERENCES: 

 

Recommended Readings:

  • Plant Biotechnology: Principles and Applications Malik ZainulAbdin (Editor), Usha Kiran (Editor), Kamaluddin (Editor), Athar Ali 1st Edition. Publishers: Springer. 2018
  • Plant, Genes and Crop Biotechnology.  Marten J. Chrispeels& David E. Sadava:  2nd Ed. Jones and Bartlett Publishers. 2002.
  • Biotechnologies of Crop Improvement, Volume 2: Transgenic Approaches. Satbir Singh Gosal (Editor), Shabir Hussain Wani  (Editor). Publishers Springer. 2018
  • Practical Application of plant Molecular Biology. R.J. Henry: Chapman and hall.1997.
  • Laboratory Manual for Plant Biotechnology. H.S Chawla: Oxford & IBH publishing Co. Pvt Ltd. 2003
  • Plant Tissue Culture – Techniques &Experiments Roberta. H. Smith: 2nd ed. Elsevier. 2000
  • Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler:  Oxford University Press. 2005
  • Plant Tissue Culture. S.S. Bhojwani and M.K Razdan:  Elsevier. 2004
  • Plant Biotechnology. J. Hammond, P. Mc Garvey & V. Yusibov:  Springer (India) Pvt Ltd. 2000
  • Biotechnology – Secondary Metabolites. K.G. Ramawat, J.M Merllon:  Oxford & IBH publishing house Co. Pvt. Ltd. 2000

 

e RESOURCES:

 

JOURNALS:

  • Plant Biotechnology Journal - Wiley Online Library
  • Plant Science Journal Science Direct.com by Elsevier
  • International Journal of Plant Scirnces (PCTOC)- Springer
  • Plant Tissue Culture – MDPI
  • Plant Tissue Culture and Biotechnology - Bangladesh

 

 

 

 

 

 

Academic Year: 
2022-2023 [11]

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Source URL: https://www.biotech.iisuniv.ac.in/courses/subjects/plant-tissue-culture-5

Links:
[1] https://www.biotech.iisuniv.ac.in/courses/subjects/plant-tissue-culture-5
[2] https://www.agrifarming.in/tissue-culture-plants-techniques-for-beginners?msclkid=db5540f3b3e411ec8e60c4a9f1e82a2b
[3] https://pubmed.ncbi.nlm.nih.gov/23090683/
[4] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC150518/?msclkid=0397ec64b3e511ec8d07d330259cf542
[5] https://link.springer.com/chapter/10.1007/978-3-642-84306-8_10?msclkid=2df13c6bb3e511ec84ffd2f1257e84bf
[6] https://indiabioscience.org/media/articles/DBT-Life-Science-Protocol-Manual.pdf
[7] https://experiments.springernature.com/articles/10.1385/0-89603-127-6:499
[8] https://www.biologydiscussion.com/plants/plant-tissue-culture/experiments-on-plant-tissue-culture/57409
[9] https://www.cambridge.org/ca/9780521478922
[10] https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793(86)80556-7
[11] https://www.biotech.iisuniv.ac.in/academic-year/2022-2023